Molecular Biology

QiKai Gene provides a comprehensive range of raw materials and reagents for molecular biology experiments. Our portfolio includes common PCR, quantitative real-time PCR, high-fidelity PCR, isothermal PCR, and other key reagents, as well as essential enzymes such as Taq polymerase, high-fidelity enzymes, and reverse transcriptase.

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Molecular Biology

Product Introduction

  QKGEN® Universal Blue qPCR Master Mix(Dye Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color,and has the effect of sample addition tracing. The core component QKGEN® Taq DNA polymerase uses antibody hot start to effectively inhibit non- specific amplification due to primer annealing during sample preparation. At the same time, the formula adds thee promoting factors to improve the amplification efficiency of PCR reaction and equalize the amplification of genes with different GC contents(30~70%), so that quantitative  PCR can obtain a good linear relationship in a wide quantitative region. This product contains special ROX Passive Reference Dye, which is applicable to all qPCR instruments. It is not necessary to adjust the concentration of ROX on different instruments. It is only necessary to add primers and templates to prepare the reaction system for amplification.