The QKGEN® probe design platform is a probe design scheme based on multi-factor algorithms and employing the "perception algorithm". It conducts multi-layer probe design for the target area. The probes use biotin-labeled NTP and nucleic acid analogues as synthetic raw materials. The prepared specific probes can undergo rapid liquid-phase hybridization with the target genomic library. After the hybridization is completed, streptavidin magnetic beads are used to bind the probes and the hybridization products of the target area library, and the obtained target area library is enriched and sequenced.

   This platform is designed and optimized based on thermodynamic stability and AI machine learning. It fully considers the balance between the coverage and capture efficiency of repetitive sequence regions, enabling customized design for any species and any region, and ensuring precise and efficient design.

   QKGEN® Custom's Probes Customization Solution is a flexible and user-friendly one-stop service provided by QiKai Gene, which designs capture probe groups based on the areas of interest of the customers. It covers the entire process from probe design, production synthesis, quality inspection, and optimization. Through strict quality control and optimized NGS QC services, it can provide customers with high-quality customized hybrid capture solutions, covering regions such as coding regions, non-coding regions, point mutations (SNV), insertions/deletions (indel), copy number variations (CNV), microsatellite instability (MSI), structural variations, etc.

    2. Provide higher coverage uniformity and capture efficiency.

    3. Autonomous memory function: By importing the target area, it can identify whether there are complex and difficult-to-capture areas. For these areas, they can be optimized separately, or exclusion suggestions can be provided autonomously. This reduces the difficulty and intensity of probe design and saves design time.

Note: Green indicates retention of the probe, Yellow indicates a risk probe, and Red indicates a probe that is recommended to be removed.

     4. A strict quality control system ensures that each probe is independently synthesized and quality-checked, resulting in high accuracy and stable batches.

     5. flexible customization, Spike-in a variety of applications at any time.

     6. Once customized, Qikai Gene can provide a complete set of targeted capture solutions.

Probe customization process: