Molecular Biology
QiKai Gene provides a comprehensive range of raw materials and reagents for molecular biology experiments. Our portfolio includes common PCR, quantitative real-time PCR, high-fidelity PCR, isothermal PCR, and other key reagents, as well as essential enzymes such as Taq polymerase, high-fidelity enzymes, and reverse transcriptase.
Product Introduction
QKGEN® HotStart DNA Polymerase is a novel hot-start enzyme. It uses two proprietary DNA binding proteins that at room temperature bind to the double-strand DNA template and primer, effectively neutralizing the DNA polymerase activity until denaturation occurs. As the denaturation step proceeds, the two proteins are inactivated, and the released primers and templates participate in the amplification reaction, enhancing PCR amplification efficiency.
Highlights
·Fidelity is 18 times that of QKGEN® Taq DNA Polymerase.
·Extension rate is about 1-2 kb/min.
·Specificity is superior to antibody blocking and chemical blocking of hot-start DNA polymerases.
·Prepare reactions at room temperature to reduce non-specific amplification and primer dimers.
·The use of Taq antibodies reduces the risk of potential DNA contamination from mammals.
·Unlike chemically modified Taq, no heating steps are required.
·Amplification of genomic DNA fragments(≤15kb).
Unit Definition
One unit of QKGEN® HotStart DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
·Functional absence of double-and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.
·Each batch of the HotStart Taq DNA Polymerase has been assayed for amplification efficiency of the p53 gene from 10ng of human genomic DNA.
Components
|
Name |
QAP141-1 |
QAP141-2 |
QAP141-3 |
|
HotStart DNA Polymerase (2.5 U/μL) |
500U × 1 |
500U × 2 |
500U × 6 |
|
10 × HotStart Buffer |
1.2 mL × 2 |
1.2 mL × 4 |
1.2 mL × 12 |
|
2.5 mM dNTPs |
800 μL × 2 |
800 μL × 4 |
1.2 mL × 8 |
|
10×GC Enhancer |
400 μL |
800 μL |
1.0 mL |
|
6 × DNA Loading Buffer |
1 mL |
1 mL |
1 mL × 2 |
Address：Room 908, Building A, No.3 Juquan Road, Huangpu District, Guangzhou
Email：hr@qk-bio.com
Tel：020-32037601
