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Molecular Biology

QiKai Gene provides a comprehensive range of raw materials and reagents for molecular biology experiments. Our portfolio includes common PCR, quantitative real-time PCR, high-fidelity PCR, isothermal PCR, and other key reagents, as well as essential enzymes such as Taq polymerase, high-fidelity enzymes, and reverse transcriptase.

Home / Molecular Biology / Enzymes / High-Fidelity DNA Polymerase / QKGEN® High-Fidelity DNA Polymerase

Molecular Biology

Enzymes
Taq DNA Polymerase High-Fidelity DNA Polymerase HotStart DNA Polymerase Others
PCR & qPCR
Conventional PCR Ready Mix High-fidelity PCR mix qPCR Mix
RT-PCR & qRT-PCR
Electrophoresis
Nucleic acid extraction and purification
Product description Order information Download

Product Introduction

QKGEN® High-Fidelity DNA Polymerase based on Pyrococcus Furiosis DNA Polymerase, genetically engineered. This enzyme possesses 5’→3’ DNA polymerase activity and 3’→5’ exonuclease activity, with fidelity 83 times that of Taq DNA polymerase and 9 times that of ordinary Pfu DNA polymerase. The enzyme solution contains two  monoclonal  antibodies that  inhibit  polymerase  activity  and  3’→5’ exonuclease activity at  room temperature, enabling simple and highly specific Hot Start PCR, significantly improving the detection rate and specificity of the amplified products. The enzyme solution contains extension factors, enabling the enzyme to amplify long fragments, with the length of the amplified target fragment reaching up to 13 kb. This product is equipped with an optimized buffer, making the enzyme suitable for the amplification of complex templates. The amplified products have blunt ends.

Components

Components No.

Name

Q10153-1

Q10153-2

Q10153-3

Q10153-4

10153-A

QKGEN® High-Fidelity DNA Polymerase (1 U/μL)

250 μL

500 μL

500 μL×2

500μL×4

10153-B

2× Plus PCR buffer

(Contains Mg2+, dNTPs)

6.25 mL

12.5 mL

25 mL

50  mL

10153-C

6 × DNA Loading Buffer

3.0 mL

6.0 mL

12 mL

24 mL



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Email：hr@qk-bio.com

Tel：020-32037601

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