Molecular Biology
QiKai Gene provides a comprehensive range of raw materials and reagents for molecular biology experiments. Our portfolio includes common PCR, quantitative real-time PCR, high-fidelity PCR, isothermal PCR, and other key reagents, as well as essential enzymes such as Taq polymerase, high-fidelity enzymes, and reverse transcriptase.
Product Introduction
QKGEN® Taq DNA Polymerase for PAGE is purified from E.coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94kDa. This enzyme has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity, but lacks 3'-5' exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
Highlights
·Extension rate is about 1-2 kb/min.
·Unique buffer system compatible with PAGE.
·Template-independent “A” can be generated at the 3'end of the PCR product. PCR products can be directly cloned into pEASY-T vectors.
·Amplification of genomic DNA fragment up to 3 kb.
Unit Definition
One unit of Taq DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
Taq DNA Polymerase for PAGE has passed the following quality control assays:
functional absence of double-and single-strand endonuclease activity; >99%homogeneous measured by SDS-PAGE.
Each batch of Taq DNA Polymerase for PAGE has been assayed for amplification efficiency to amplify p53 gene from 10ng of human genomic DNA.
Storage Buffer
20 mM Tris-HCl(pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers.
Components
|
Name |
QAP111-1 |
QAP111-2 |
|
Taq DNA Polymerase for PAGE (5 U/μL) |
2500U |
2500U × 4 |
|
10 × Taq Buffer for PAGE |
1.2 mL × 5 |
1.2mL × 20 |
|
2.5mM dNTPs |
800 μL × 5 |
800 μL × 20 |
|
6 × DNA Loading Buffer |
1 mL |
1 mL × 4 |
Address：Room 908, Building A, No.3 Juquan Road, Huangpu District, Guangzhou
Email：hr@qk-bio.com
Tel：020-32037601
