Product

Extraction Kit DNA Library Preparation RNA Library Preparation Liquid Hybridization Adapters Others

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Product Introduction

QKGEN® Transposase Rapid DNA Library Prep kit is a rapid DNA library construction kit based on a novel transposase system. The transposition enzyme library construction scheme adopted by the kit uses A simple enzymatic reaction to replace the steps of fragment, terminal patch and A and splicing in the conventional DNA library construction process. After splicing, there is no need for purification, and only a new tube is needed for PCR amplification, and then purification or fragment screening is performed. Greatly simplify the operation process and shorten the library construction time, and the construction time of a single library is not more than 60 minutes. Moreover, in the sample input range of 1-50 ng, without considering the input amount of template, the optimized kit system can meet the needs of using only the same set of reagents and the same process to complete the library construction, ensuring the efficiency and stability of library construction.

Scope of application

1. Library construction of 1ng~50ng double-stranded DNA
2. Low starting amount of trace samples, such as pathogenic microorganism detection.
3. Rapid DNA library construction of human genome/large genome/complex genome;
4. Rapid DNA library construction for small genome/microorganism/plasmid;
5. Suitable for rapid library construction of PCR amplification products;
6. It can be applied to chromatin open region research, genetic variation, transcriptome, epigenetics, pathogenic microorganism detection, plant and animal breeding and other directions.

Product Advantages

1. Simplicity. One step to complete fragmentation, end repair plus A and add joint.
2. Be quick. No purification is required after joint connection, and the preparation of a single sample library does not exceed 60min.
3, suitable for precious samples. The minimum sample input is only 1ng.
4, strong compatibility. The optimized system can be compatible with 1-50ng DNA inputs at the same time, and the same system does not require separate management and specification operations.