1. Be quick. Compared with the traditional two-step method, the database construction time of a single sample is at least 1h shorter.

2. Low preference, comparable to mechanical interruption. Under the same experimental conditions, the GC content of the libraries constructed by QKGEN® one-step method and conventional two-step method after ultrasonic interruption tend to be flat and almost have no fluctuation.

Figure 1. QKGEN® Enzyme Plus DNA Library Prep Kit Breaks DNA (left) and Covaris Ultrasound Interrupter Breaks DNA mechanically (right)