QKGEN® High-Fidelity DNA Polymerase based on Pyrococcus Furiosis DNA Polymerase, genetically engineered. This enzyme possesses 5’→3’ DNA polymerase activity and 3’→5’ exonuclease activity, with fidelity 83 times that of Taq DNA polymerase and 9 times that of ordinary Pfu DNA polymerase.
Advantage:
1.The enzyme solution contains two monoclonal antibodies that inhibit polymerase activity and 3’→5’ exonuclease activity at room temperature, enabling simple and highly specific Hot Start PCR, significantly improving the detection rate and specificity of the amplified products.
2.Contains extension factors, enabling the enzyme to amplify long fragments, with the length of the amplified target fragment reaching up to 13 kb.
3.This product is equipped with an optimized buffer, making the enzyme suitable for the amplification of complex templates. The amplified products have blunt ends.
Application:
1.Gene cloning;
2.Complex DNA template amplification;
3.High-throughput library construction.
Components:
Storage:
Transport with ice packs.This product should be stored at -25~-15℃ for 12 months.
Recommended PCR reaction system:
For detailed information and operation, please refer to the manual.
NOTES:
1. For your safety and health, please wear a lab coat and disposable gloves when operating.
2. This product is for scientific research purposes only!