Product

Extraction Kit DNA Library Preparation RNA Library Preparation Liquid Hybridization Adapters Others PCR&qPCR Master Mix

Product Description Product Category

Product Introduction

QKGEN® 2× PCR Master Mix(No Dye) is a kind of conventional PCR premixed solution which is ready to use,including Taq DNA Polymerase, dNTP mix, MgCl and optimized buffer.

Advantages:

1.Only the primer and template can be added for amplification, which greatly simplifies the operation steps of experiment.

2.Contains excellent stabilizers and can be stored for 3 months at 4℃.

3.The PCR product have 3'-dA protrusion and can be easily cloned into T vector.

Storage:

The product should be stored at -25℃ ~ -15℃ for 2 years.

Instructions:

[Note]:

a)Template usage:50-200 ng genomic DNA; 0.1-10 ng plasmid DNA.

b)Annealing temperature(★):Please refer to the theoretical Tm value of primers.The annealing temperature can be set to 2-5℃ lower than the theoretical value of the primer.

c)Extention time:For molecular identification, 30 sec/kb is recommended. For gene cloning, 60 sec/kb is recommended.

NOTES:

1. PCR products with 2× PCR Master Mix are not suitable for polyacrylamide gel electrophoresis.

2. For your safety and health,please wear lab coats and disposable gloves for operation!

3. This product is for research use ONLY!