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Extraction Kit DNA Library Preparation RNA Library Preparation Liquid Hybridization Adapters Others

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Background Introduction

Fungal ITS sequencing refers to sequencing the Internal Transcribed Spacer (ITS) of the fungus. The ITS region is located between the 18S, 5.8S and 28S rRNA genes of fungi and is a non-coding region of ribosomal DNA. In the evolutionary process, ITS regions are relatively consistent within species, and the differences between species are obvious, which can reflect the differences between species and even strains. Therefore, by sequencing and analyzing ITS sequences, fungal species can be identified and classified effectively.

Product Introduction

This kit designed specific amplification primers for the ITS1 and ITS2 regions of fungi. First, the ITS1 and ITS2 regions of fungi were specifically amplified, and at the same time, the common sequence of the next PCR was amplified. In the second step, PCR amplified sequencing joints such as sequencing primers and sample tags to construct a complete illumina library. This method has simple steps and is easier to realize automatic library construction. The target area of this kit is approximately 200-600bp, and sequencing using the illumina Miseq platform PE300 or the Novaseq PE250 strategy is recommended.

Product Features

1. Suitable for library construction of 1-10ng fungal DNA
2. Highly efficient expansion of the variability area, the entire library construction process only takes 2-3 hours
3. The abundance and diversity of highly specific primers were high
4. Can be customized, can be customized according to the needs of other variation areas or other platforms such as MGI platform library

Product Applications