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Extraction Kit DNA Library Preparation RNA Library Preparation Liquid Hybridization Adapters Others

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Product Introduction

This kit is a second-generation sequencing library preparation kit specifically designed for the V3-V4 region of bacterial 16S rDNA for use on the illumina platform. This kit designs specific amplification primers for the V3-V4 region of bacterial 16S rRNA. First, the V3-V4 region of bacterial 16S rRNA is specifically amplified, and at the same time, the common sequence of the next PCR is amplified. The sequencing primer, sample label and other sequencing connector parts are amplified by PCR in the second step. You can build a complete illumina library. This method has simple steps and is easier to realize automatic library construction. The target area of this kit is approximately 450bp, and sequencing using the illumina Miseq platform PE300 or Novaseq PE250 strategy is recommended.

Library Construction Principle