Product

Extraction Kit DNA Library Preparation RNA Library Preparation Liquid Hybridization Adapters Others

Product Description Product Performance Order Information Download

Product Introduction

Compared with the traditional two-step method of library construction, this kit uses high-quality fragment enzyme, without complicated ultrasonic interruption process, and combines the end repair and the "A" tail addition module into one, so that the fragment/end repair/addition of "A" can be carried out in the same system. The optimized system makes the reaction products do not need to be purified by magnetic beads, and can be directly connected. It greatly shortens the time of building the DNA library, simplifies the process of building the DNA library, and makes the DNA library construction more efficient and simple. The fragment enzyme used in this kit can randomly cut double-stranded DNA, and the bias of the fragment cut by the enzyme is very low, which is comparable to the fragment interrupted by ultrasound.

Product Features

1. 1ng to 1µg double-stranded DNA, FFPE >50ng, amplicon >10ng.
2. Applicable to different species sources, including: various prokaryotic or eukaryotic microbial genomes, animal and plant genomes, including human and mouse genomes, etc.
3. Compatible with the following sample types: genomic DNA, FFPE, ChIP DNA, double-stranded cDNA products and PCR amplification products.
4. It can be applied to whole genome sequencing, targeted capture sequencing, PCR-free sequencing, immunoprecipitation sequencing, etc.
5. Library construction compatible with long and short joints.
6. Library construction compatible with illumina and BGI high-throughput sequencing platforms.

Product advantages

1. The process is simpler than the conventional two-step method
2. The building time is shorter, from 3-3.5h to 2-2.5 hours
3. No need to rely on expensive mechanical interruption instruments, saving costs
4. Lower preference, comparable to ultrasonic interruption
5. Strong applicability, easily cope with 1ng-1ug, different species, different types, with or without EDTA DNA
6. Easier to match automated workflows