1. Excellent on-target rate

Figure 1. Genome alignment rate and target rate. Three 100ng human blood genomes were prepared, ultrasonically interrupted on the Covaris interrupter, then the libraries were constructed with the QKGEN® DNA Library Construction Kit (for Illumina), and hybridized and captured in three batches of QKGEN® EXome Panel V1.0, respectively. The sequencing mode was Illumina Novaseq, PE150, and the reference genome hg38 was compared by BWA, and the on-target was calculated according to the number of reads.

2. higher coverage uniformity

Figure 2. Indicators of coverage uniformity. Three 100ng human blood genomes were prepared, ultrasonically interrupted on the Covaris interrupter, then the libraries were constructed with the QKGEN® DNA Library Construction Kit (for Illumina), and hybridized and captured in three batches of QKGEN® EXome Panel V1.0, respectively. The sequencing mode was Illumina Novaseq, PE150, and the reference genome hg38 was compared by BWA, and the on-target was calculated according to the number of reads.

Figure 3. Coverage by multiple. Three 100ng human blood genomes were prepared, ultrasonically interrupted on the Covaris interrupter, then the libraries were constructed with the QKGEN® DNA Library Construction Kit (for Illumina), and hybridized and captured in three batches of QKGEN® EXome Panel V1.0, respectively. The sequencing mode was Illumina Novaseq, PE150, and the reference genome hg38 was compared by BWA, and the on-target was calculated according to the number of reads.