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Extraction Kit DNA Library Preparation RNA Library Preparation Liquid Hybridization Adapters Others

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1. Fast, the shortest hybridization time can be as low as 1 hour

2. The data of different cross multiplicities showed excellent performance

Figure 1. QKGEN® DNA rapid Hybridization capture kit performance on different hybridization multiplicities. The QKGEN® DNA rapid hybridization capture kit and the T Company customer panel with the size of 2Mb were used for 500ng/ library input 1-plex hybridization and 250ng/ library input 8plex and 16-plex multiple hybridization capture with the hybridization time of 2 hours. Finally, Illumina NoveSeqTM PE150 was used for sequencing.

3. High uniformity of coverage

Figure 2. Performance of QKGEN® DNA rapid hybridization capture kit on coverage uniformity. The 8plex multiple hybridization capture with 200ng/ library input was performed using the T Company rapid hybridization and elution kit, QKGEN® DNA rapid hybridization capture kit and the T Company customer panel with the size of 2Mb. The hybridization time was 2 hours. And 16plex multiple hybridization capture with 200ng/ library input with the T Company rapid hybridization and elution kit and QKGEN® DNA rapid hybridization capture kit and the T Company customer panel with the size of 2Mb respectively. The hybridization time is 2 hours. Finally, Illumina NoveSeqTM PE150 was used for sequencing. The results showed that there was no significant difference in coverage uniformity between QKGEN® DNA rapid hybridization capture kit and T's rapid hybridization and elution kit.

4. Excellent capture efficiency

Figure 3. Performance of QKGEN® DNA rapid Hybridization capture kit on target rate. The 8plex multiple hybridization capture with 200ng/ library input was performed using the T Company rapid hybridization and elution kit, QKGEN® DNA rapid hybridization capture kit and the T Company customer panel with the size of 2Mb. The hybridization time was 2 hours. And 16plex multiple hybridization capture with 200ng/ library input with the T Company rapid hybridization and elution kit and QKGEN® DNA rapid hybridization capture kit and the T Company customer panel with the size of 2Mb respectively. The hybridization time is 2 hours. Finally, Illumina NoveSeqTM PE150 was used for sequencing. The results showed that the QKGEN® DNA rapid hybridization capture kit had a better hit rate than T's rapid hybridization and elution kit.

5. There was no difference in performance among different sequencing platforms

Figure 4. Comparison of QKGEN® DNA rapid Hybridization capture kit performance on two sequencing platforms. QKGEN® DNA Rapid Hybridizer capture Kit, QKGEN® Universal Blocker (for illumina), QKGEN® MGI-SI Universal Blockers, and a 2Mb T Company customer were used The panel conducted 8plex multiple hybridization capture with 200ng/ library input, and the hybridization time was 2 hours. Finally, Illumina NoveSeqTM PE150 and DNBSEQ-T7, PE150 were sequenced, respectively. The results showed that when QKGEN® DNA rapid hybridization capture kit was used for hybridization capture of different sequencing platforms, there was no significant difference in the capture data comparison rate and capture efficiency.