1. 1-10ng low starting amount, suitable for a variety of sampling methods to extract trace bacterial DNA

2. Excellent sequencing data. Six sputum samples were used, 1-10ng bacterial DNA was input, library construction was performed using QKGEN® bacterial 16S rDNA V3-V4 regional library construction kit, and high-throughput sequencing was performed by PE250 strategy, with Q30 >98%.

3. High richness and diversity, covering more than 85% of species.

Figure 1: Alpha diversity in 6 sputum samples

Figure 2：Venn Figure

Figure 3: Heat map by genus, covering the abundance of the Top 50 species

Figure 4: Phylogenetic tree. The abundance of the Top50 species of 6 sputum samples was covered