Product

Extraction Kit DNA Library Preparation RNA Library Preparation Liquid Hybridization Adapters Others

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Product Introduction

This kit is a second-generation sequencing library preparation kit specifically designed for the V3-V4 region of bacterial 16S rDNA for use on the illumina platform. A complete illumina library was constructed by specific amplification of the V3-V4 region of 16S rDNA with this reagent, followed by terminal repair, "A" tail addition and splice connection. The target area of this kit is approximately 450bp, and sequencing using the illumina Miseq platform PE300 or Novaseq PE250 strategy is recommended.

Product Features

1. Suitable for library construction of 1-10ng pure bacterial DNA
2. Amplification method, the time of building a single sample is about 3.5 hours
3. High-fidelity amplification reagent, efficient amplification of 16S rDNA V3-V4 region
4. The abundance and diversity of highly specific primers were high
5. Can be customized, can be customized according to the needs of other variation areas or other platforms such as MGI platform library
6. Two-step expansion can be customized to simplify the process and make it easier to automate

Library Construction Principle