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Extraction Kit DNA Library Preparation RNA Library Preparation Liquid Hybridization Adapters Others

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QKGEN® SYBR QPCR Master mix is a 2× concentration premixed solution for real-time fluorescent quantitative PCR amplification. Mix contains hot start DNA polymerase, SYBR Green I, dNTPs, Mg 2+, which can be selected by High ROX, Low ROX and without ROX according to the model of fluorescence quantitative PCR instrument. When used, only templates and primers can be added to the amplification system for real-time fluorescence quantitative PCR, which greatly simplifies the operation process and reduces the probability of contamination.
The DNA polymerase ligand used in this product can adjust the DNA polymerase activity in real time with the change of temperature. The formulation added factors that effectively inhibit the amplification of non-specific PCR and enhance the amplification efficiency of PCR reaction, so that quantitative PCR can obtain a good linear relationship in a wide quantitative region.