Molecular Biology
QiKai Gene provides a comprehensive range of raw materials and reagents for molecular biology experiments. Our portfolio includes common PCR, quantitative real-time PCR, high-fidelity PCR, isothermal PCR, and other key reagents, as well as essential enzymes such as Taq polymerase, high-fidelity enzymes, and reverse transcriptase.
Product Introduction
QKGEN® Taq DNA Polymerase is isolated from E.coli expressing a cloned DNA polymerase gene from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94kDa. The Taq DNA Polymerase has 5'→3' DNA polymerase and exonuclease activity and is suitable for routine polymerase chain reaction (PCR) applications. Please note, PCR products using this enzyme are not suitable for polyacrylamide gel electrophoresis(PAGE).
Highlights
·Extension rate is about 1-2 kb/min.
·Amplification of genomic DNA fragment up to 4 kb
Unit Definition
One unit of Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.
Quality Control
·Functional absence of double-and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE.
·Each batch of Taq DNA Polymerase has been assayed for amplification efficiency of the p53 genefrom 10ng of human genomic DNA.
Storage Buffer
20 mM Tris-HCl(pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers
Components
|
Name |
QAP111-1 |
QAP111-2 |
QAP111-3 |
|
Taq DNA Polymerase (5 U/μL) |
500U × 1 |
500U × 6 |
2500U × 4 |
|
10 × Taq Buffer |
1.2 mL × 1 |
1.2 mL × 6 |
1.2 mL × 20 |
|
2.5 mM dNTPs |
800 μL |
800 μL × 6 |
800 μL × 20 |
|
6 × DNA Loading Buffer |
1 mL |
1 mL × 2 |
1 mL × 4 |
Address：Room 908, Building A, No.3 Juquan Road, Huangpu District, Guangzhou
Email：hr@qk-bio.com
Tel：020-32037601
